Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
15 |
2.7% |
| Arg[R] |
19 |
3.4% |
| Asn[N] |
31 |
5.6% |
| Asp[D] |
34 |
6.1% |
| Cys[C] |
7 |
1.3% |
| Gln[Q] |
22 |
3.9% |
| Glu[E] |
41 |
7.3% |
| Gly[G] |
29 |
5.2% |
| His[H] |
16 |
2.9% |
| Ile[I] |
42 |
7.5% |
| Leu[L] |
63 |
11.3% |
| Lys[K] |
38 |
6.8% |
| Met[M] |
7 |
1.3% |
| Phe[F] |
37 |
6.6% |
| Pro[P] |
17 |
3.0% |
| Ser[S] |
47 |
8.4% |
| Thr[T] |
24 |
4.3% |
| Trp[W] |
13 |
2.3% |
| Tyr[Y] |
22 |
3.9% |
| Val[V] |
34 |
6.1% |
| Total Length |
558 |
|
| Instability index (II) |
38.34 |
| This classifies the protein as stable |
|
| Carbon |
2968 |
| Hydrogen |
4521 |
| Nitrogen |
751 |
| Oxygen |
855 |
| Sulfur |
14 |
| Total number of atoms |
9109 |
| Formula = C2968H4521N751O855S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
104655 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
104280 M-1 cm-1 |
| assuming all Cys residues are reduced |
104280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
104655 M-1 cm-1 |
|
|
| Codon Bias |
-0.053 |
| Codon Adaptation Index |
0.114 |
| Frequency of Optimal Codons |
0.388 |
| Hydropathicity of Protein |
-0.240 |
| Aromaticity Score |
0.129 |
|
