ZRT1/YGL255W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 26 6.9%
Arg[R] 13 3.5%
Asn[N] 14 3.7%
Asp[D] 18 4.8%
Cys[C] 6 1.6%
Gln[Q] 8 2.1%
Glu[E] 14 3.7%
Gly[G] 34 9.0%
His[H] 7 1.9%
Ile[I] 23 6.1%
Leu[L] 37 9.8%
Lys[K] 12 3.2%
Met[M] 7 1.9%
Phe[F] 25 6.6%
Pro[P] 12 3.2%
Ser[S] 30 8.0%
Thr[T] 29 7.7%
Trp[W] 11 2.9%
Tyr[Y] 16 4.3%
Val[V] 34 9.0%
Total Length 376

Instability index
Instability index (II) 23.75
This classifies the protein as stable

Atomic composition
Carbon 1915
Hydrogen 2894
Nitrogen 474
Oxygen 538
Sulfur 13
Total number of atoms 5834
Formula = C1915H2894N474O538S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 84715 M-1 cm-1
assuming NO Cys residues appear as half cystines 84340 M-1 cm-1
assuming all Cys residues are reduced 84340 M-1 cm-1
assuming all pairs of Cys residues form cystines 84715 M-1 cm-1

Aliphatic index
Aliphatic index 95.37

Coding Region Translation Calculations
Codon Bias 0.316
Codon Adaptation Index 0.255
Frequency of Optimal Codons 0.589
Hydropathicity of Protein 0.286
Aromaticity Score 0.138