RAI1/YGL246C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 19 4.9%
Arg[R] 22 5.7%
Asn[N] 21 5.4%
Asp[D] 24 6.2%
Cys[C] 7 1.8%
Gln[Q] 11 2.8%
Glu[E] 34 8.8%
Gly[G] 22 5.7%
His[H] 8 2.1%
Ile[I] 26 6.7%
Leu[L] 39 10.1%
Lys[K] 32 8.3%
Met[M] 2 0.5%
Phe[F] 22 5.7%
Pro[P] 11 2.8%
Ser[S] 27 7.0%
Thr[T] 20 5.2%
Trp[W] 4 1.0%
Tyr[Y] 15 3.9%
Val[V] 21 5.4%
Total Length 387

Instability index
Instability index (II) 36.92
This classifies the protein as stable

Atomic composition
Carbon 1997
Hydrogen 3121
Nitrogen 537
Oxygen 598
Sulfur 9
Total number of atoms 6262
Formula = C1997H3121N537O598S9

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 44725 M-1 cm-1
assuming NO Cys residues appear as half cystines 44350 M-1 cm-1
assuming all Cys residues are reduced 44350 M-1 cm-1
assuming all pairs of Cys residues form cystines 44725 M-1 cm-1

Aliphatic index
Aliphatic index 86.15

Coding Region Translation Calculations
Codon Bias 0.045
Codon Adaptation Index 0.137
Frequency of Optimal Codons 0.438
Hydropathicity of Protein -0.463
Aromaticity Score 0.106