Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
7.3% |
| Arg[R] |
36 |
5.1% |
| Asn[N] |
36 |
5.1% |
| Asp[D] |
64 |
9.0% |
| Cys[C] |
7 |
1.0% |
| Gln[Q] |
16 |
2.3% |
| Glu[E] |
44 |
6.2% |
| Gly[G] |
36 |
5.1% |
| His[H] |
14 |
2.0% |
| Ile[I] |
48 |
6.8% |
| Leu[L] |
60 |
8.5% |
| Lys[K] |
72 |
10.2% |
| Met[M] |
16 |
2.3% |
| Phe[F] |
28 |
4.0% |
| Pro[P] |
30 |
4.2% |
| Ser[S] |
28 |
4.0% |
| Thr[T] |
36 |
5.1% |
| Trp[W] |
12 |
1.7% |
| Tyr[Y] |
23 |
3.2% |
| Val[V] |
50 |
7.1% |
| Total Length |
708 |
|
| Instability index (II) |
31.45 |
| This classifies the protein as stable |
|
| Carbon |
3628 |
| Hydrogen |
5734 |
| Nitrogen |
980 |
| Oxygen |
1064 |
| Sulfur |
23 |
| Total number of atoms |
11429 |
| Formula = C3628H5734N980O1064S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
100645 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
100270 M-1 cm-1 |
| assuming all Cys residues are reduced |
100270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
100645 M-1 cm-1 |
|
|
| Codon Bias |
0.478 |
| Codon Adaptation Index |
0.461 |
| Frequency of Optimal Codons |
0.694 |
| Hydropathicity of Protein |
-0.458 |
| Aromaticity Score |
0.089 |
|
