EMC4/YGL231C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 13 6.8%
Arg[R] 4 2.1%
Asn[N] 12 6.3%
Asp[D] 4 2.1%
Cys[C] 0 0.0%
Gln[Q] 17 8.9%
Glu[E] 6 3.2%
Gly[G] 11 5.8%
His[H] 2 1.1%
Ile[I] 15 7.9%
Leu[L] 16 8.4%
Lys[K] 13 6.8%
Met[M] 10 5.3%
Phe[F] 7 3.7%
Pro[P] 11 5.8%
Ser[S] 17 8.9%
Thr[T] 12 6.3%
Trp[W] 5 2.6%
Tyr[Y] 8 4.2%
Val[V] 7 3.7%
Total Length 190

Instability index
Instability index (II) 49.91
This classifies the protein as unstable

Atomic composition
Carbon 969
Hydrogen 1513
Nitrogen 253
Oxygen 277
Sulfur 10
Total number of atoms 3022
Formula = C969H1513N253O277S10

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 39420 M-1 cm-1

Aliphatic index
Aliphatic index 81.16

Coding Region Translation Calculations
Codon Bias 0.099
Codon Adaptation Index 0.139
Frequency of Optimal Codons 0.469
Hydropathicity of Protein -0.267
Aromaticity Score 0.105