Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
4.1% |
| Arg[R] |
41 |
4.3% |
| Asn[N] |
88 |
9.2% |
| Asp[D] |
98 |
10.2% |
| Cys[C] |
7 |
0.7% |
| Gln[Q] |
31 |
3.2% |
| Glu[E] |
72 |
7.5% |
| Gly[G] |
55 |
5.7% |
| His[H] |
11 |
1.1% |
| Ile[I] |
52 |
5.4% |
| Leu[L] |
88 |
9.2% |
| Lys[K] |
57 |
5.9% |
| Met[M] |
20 |
2.1% |
| Phe[F] |
36 |
3.8% |
| Pro[P] |
28 |
2.9% |
| Ser[S] |
98 |
10.2% |
| Thr[T] |
41 |
4.3% |
| Trp[W] |
7 |
0.7% |
| Tyr[Y] |
38 |
4.0% |
| Val[V] |
51 |
5.3% |
| Total Length |
958 |
|
| Instability index (II) |
39.95 |
| This classifies the protein as stable |
|
| Carbon |
4697 |
| Hydrogen |
7308 |
| Nitrogen |
1286 |
| Oxygen |
1595 |
| Sulfur |
27 |
| Total number of atoms |
14913 |
| Formula = C4697H7308N1286O1595S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
95495 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
95120 M-1 cm-1 |
| assuming all Cys residues are reduced |
95120 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
95495 M-1 cm-1 |
|
|
| Codon Bias |
0.089 |
| Codon Adaptation Index |
0.170 |
| Frequency of Optimal Codons |
0.465 |
| Hydropathicity of Protein |
-0.704 |
| Aromaticity Score |
0.085 |
|
