Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
5.6% |
| Arg[R] |
53 |
6.6% |
| Asn[N] |
54 |
6.7% |
| Asp[D] |
44 |
5.5% |
| Cys[C] |
11 |
1.4% |
| Gln[Q] |
34 |
4.2% |
| Glu[E] |
60 |
7.5% |
| Gly[G] |
26 |
3.2% |
| His[H] |
17 |
2.1% |
| Ile[I] |
58 |
7.2% |
| Leu[L] |
79 |
9.8% |
| Lys[K] |
48 |
6.0% |
| Met[M] |
22 |
2.7% |
| Phe[F] |
25 |
3.1% |
| Pro[P] |
31 |
3.9% |
| Ser[S] |
78 |
9.7% |
| Thr[T] |
57 |
7.1% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
16 |
2.0% |
| Val[V] |
44 |
5.5% |
| Total Length |
805 |
|
| Instability index (II) |
54.65 |
| This classifies the protein as unstable |
|
| Carbon |
3961 |
| Hydrogen |
6433 |
| Nitrogen |
1137 |
| Oxygen |
1253 |
| Sulfur |
33 |
| Total number of atoms |
12817 |
| Formula = C3961H6433N1137O1253S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
40965 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
40340 M-1 cm-1 |
| assuming all Cys residues are reduced |
40340 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
40965 M-1 cm-1 |
|
|
| Codon Bias |
0.047 |
| Codon Adaptation Index |
0.118 |
| Frequency of Optimal Codons |
0.435 |
| Hydropathicity of Protein |
-0.462 |
| Aromaticity Score |
0.055 |
|
