YGL214W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 8 5.0%
Arg[R] 5 3.1%
Asn[N] 10 6.2%
Asp[D] 5 3.1%
Cys[C] 4 2.5%
Gln[Q] 8 5.0%
Glu[E] 7 4.3%
Gly[G] 2 1.2%
His[H] 5 3.1%
Ile[I] 10 6.2%
Leu[L] 18 11.2%
Lys[K] 8 5.0%
Met[M] 4 2.5%
Phe[F] 8 5.0%
Pro[P] 14 8.7%
Ser[S] 25 15.5%
Thr[T] 8 5.0%
Trp[W] 1 0.6%
Tyr[Y] 3 1.9%
Val[V] 8 5.0%
Total Length 161

Instability index
Instability index (II) 50.99
This classifies the protein as unstable

Atomic composition
Carbon 798
Hydrogen 1261
Nitrogen 213
Oxygen 240
Sulfur 8
Total number of atoms 2520
Formula = C798H1261N213O240S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 10220 M-1 cm-1
assuming NO Cys residues appear as half cystines 9970 M-1 cm-1
assuming all Cys residues are reduced 9970 M-1 cm-1
assuming all pairs of Cys residues form cystines 10220 M-1 cm-1

Aliphatic index
Aliphatic index 87.20

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000