NCS6/YGL211W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 30 8.4%
Arg[R] 22 6.1%
Asn[N] 17 4.7%
Asp[D] 19 5.3%
Cys[C] 13 3.6%
Gln[Q] 11 3.1%
Glu[E] 20 5.6%
Gly[G] 22 6.1%
His[H] 6 1.7%
Ile[I] 23 6.4%
Leu[L] 35 9.7%
Lys[K] 29 8.1%
Met[M] 8 2.2%
Phe[F] 12 3.3%
Pro[P] 9 2.5%
Ser[S] 29 8.1%
Thr[T] 16 4.5%
Trp[W] 1 0.3%
Tyr[Y] 14 3.9%
Val[V] 23 6.4%
Total Length 359

Instability index
Instability index (II) 47.54
This classifies the protein as unstable

Atomic composition
Carbon 1758
Hydrogen 2843
Nitrogen 495
Oxygen 525
Sulfur 21
Total number of atoms 5642
Formula = C1758H2843N495O525S21

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 27110 M-1 cm-1
assuming NO Cys residues appear as half cystines 26360 M-1 cm-1
assuming all Cys residues are reduced 26360 M-1 cm-1
assuming all pairs of Cys residues form cystines 27110 M-1 cm-1

Aliphatic index
Aliphatic index 89.94

Coding Region Translation Calculations
Codon Bias 0.054
Codon Adaptation Index 0.115
Frequency of Optimal Codons 0.431
Hydropathicity of Protein -0.206
Aromaticity Score 0.075