MCM6/YGL201C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 70 6.9%
Arg[R] 66 6.5%
Asn[N] 51 5.0%
Asp[D] 73 7.2%
Cys[C] 12 1.2%
Gln[Q] 39 3.8%
Glu[E] 81 8.0%
Gly[G] 61 6.0%
His[H] 17 1.7%
Ile[I] 63 6.2%
Leu[L] 74 7.3%
Lys[K] 49 4.8%
Met[M] 24 2.4%
Phe[F] 36 3.5%
Pro[P] 42 4.1%
Ser[S] 102 10.0%
Thr[T] 60 5.9%
Trp[W] 4 0.4%
Tyr[Y] 27 2.7%
Val[V] 66 6.5%
Total Length 1017

Instability index
Instability index (II) 48.06
This classifies the protein as unstable

Atomic composition
Carbon 4895
Hydrogen 7792
Nitrogen 1392
Oxygen 1605
Sulfur 36
Total number of atoms 15720
Formula = C4895H7792N1392O1605S36

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 62980 M-1 cm-1
assuming NO Cys residues appear as half cystines 62230 M-1 cm-1
assuming all Cys residues are reduced 62230 M-1 cm-1
assuming all pairs of Cys residues form cystines 62980 M-1 cm-1

Aliphatic index
Aliphatic index 78.24

Coding Region Translation Calculations
Codon Bias 0.139
Codon Adaptation Index 0.172
Frequency of Optimal Codons 0.485
Hydropathicity of Protein -0.498
Aromaticity Score 0.066