Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
218 |
8.2% |
| Arg[R] |
110 |
4.1% |
| Asn[N] |
131 |
4.9% |
| Asp[D] |
150 |
5.6% |
| Cys[C] |
26 |
1.0% |
| Gln[Q] |
87 |
3.3% |
| Glu[E] |
208 |
7.8% |
| Gly[G] |
123 |
4.6% |
| His[H] |
35 |
1.3% |
| Ile[I] |
219 |
8.2% |
| Leu[L] |
344 |
12.9% |
| Lys[K] |
173 |
6.5% |
| Met[M] |
37 |
1.4% |
| Phe[F] |
126 |
4.7% |
| Pro[P] |
101 |
3.8% |
| Ser[S] |
208 |
7.8% |
| Thr[T] |
123 |
4.6% |
| Trp[W] |
15 |
0.6% |
| Tyr[Y] |
51 |
1.9% |
| Val[V] |
187 |
7.0% |
| Total Length |
2672 |
|
| Instability index (II) |
40.37 |
| This classifies the protein as unstable |
|
| Carbon |
13362 |
| Hydrogen |
21480 |
| Nitrogen |
3478 |
| Oxygen |
3989 |
| Sulfur |
63 |
| Total number of atoms |
42372 |
| Formula = C13362H21480N3478O3989S63 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
160115 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
158490 M-1 cm-1 |
| assuming all Cys residues are reduced |
158490 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
160115 M-1 cm-1 |
|
|
| Codon Bias |
0.182 |
| Codon Adaptation Index |
0.189 |
| Frequency of Optimal Codons |
0.510 |
| Hydropathicity of Protein |
0.044 |
| Aromaticity Score |
0.072 |
|