GCN1/YGL195W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 218 8.2%
Arg[R] 110 4.1%
Asn[N] 131 4.9%
Asp[D] 150 5.6%
Cys[C] 26 1.0%
Gln[Q] 87 3.3%
Glu[E] 208 7.8%
Gly[G] 123 4.6%
His[H] 35 1.3%
Ile[I] 219 8.2%
Leu[L] 344 12.9%
Lys[K] 173 6.5%
Met[M] 37 1.4%
Phe[F] 126 4.7%
Pro[P] 101 3.8%
Ser[S] 208 7.8%
Thr[T] 123 4.6%
Trp[W] 15 0.6%
Tyr[Y] 51 1.9%
Val[V] 187 7.0%
Total Length 2672

Instability index
Instability index (II) 40.37
This classifies the protein as unstable

Atomic composition
Carbon 13362
Hydrogen 21480
Nitrogen 3478
Oxygen 3989
Sulfur 63
Total number of atoms 42372
Formula = C13362H21480N3478O3989S63

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 160115 M-1 cm-1
assuming NO Cys residues appear as half cystines 158490 M-1 cm-1
assuming all Cys residues are reduced 158490 M-1 cm-1
assuming all pairs of Cys residues form cystines 160115 M-1 cm-1

Aliphatic index
Aliphatic index 110.63

Coding Region Translation Calculations
Codon Bias 0.182
Codon Adaptation Index 0.189
Frequency of Optimal Codons 0.510
Hydropathicity of Protein 0.044
Aromaticity Score 0.072