GTS1/YGL181W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 36 9.1%
Arg[R] 27 6.8%
Asn[N] 20 5.1%
Asp[D] 30 7.6%
Cys[C] 5 1.3%
Gln[Q] 48 12.1%
Glu[E] 13 3.3%
Gly[G] 27 6.8%
His[H] 7 1.8%
Ile[I] 12 3.0%
Leu[L] 20 5.1%
Lys[K] 16 4.0%
Met[M] 10 2.5%
Phe[F] 21 5.3%
Pro[P] 15 3.8%
Ser[S] 42 10.6%
Thr[T] 14 3.5%
Trp[W] 3 0.8%
Tyr[Y] 14 3.5%
Val[V] 16 4.0%
Total Length 396

Instability index
Instability index (II) 55.39
This classifies the protein as unstable

Atomic composition
Carbon 1909
Hydrogen 2942
Nitrogen 578
Oxygen 621
Sulfur 15
Total number of atoms 6065
Formula = C1909H2942N578O621S15

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 37610 M-1 cm-1
assuming NO Cys residues appear as half cystines 37360 M-1 cm-1
assuming all Cys residues are reduced 37360 M-1 cm-1
assuming all pairs of Cys residues form cystines 37610 M-1 cm-1

Aliphatic index
Aliphatic index 52.32

Coding Region Translation Calculations
Codon Bias 0.012
Codon Adaptation Index 0.111
Frequency of Optimal Codons 0.402
Hydropathicity of Protein -0.863
Aromaticity Score 0.096