Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
6.6% |
| Arg[R] |
37 |
4.1% |
| Asn[N] |
65 |
7.2% |
| Asp[D] |
44 |
4.9% |
| Cys[C] |
12 |
1.3% |
| Gln[Q] |
36 |
4.0% |
| Glu[E] |
72 |
8.0% |
| Gly[G] |
32 |
3.6% |
| His[H] |
25 |
2.8% |
| Ile[I] |
46 |
5.1% |
| Leu[L] |
107 |
11.9% |
| Lys[K] |
68 |
7.6% |
| Met[M] |
17 |
1.9% |
| Phe[F] |
36 |
4.0% |
| Pro[P] |
35 |
3.9% |
| Ser[S] |
86 |
9.6% |
| Thr[T] |
42 |
4.7% |
| Trp[W] |
5 |
0.6% |
| Tyr[Y] |
31 |
3.5% |
| Val[V] |
42 |
4.7% |
| Total Length |
897 |
|
| Instability index (II) |
44.79 |
| This classifies the protein as unstable |
|
| Carbon |
4505 |
| Hydrogen |
7126 |
| Nitrogen |
1232 |
| Oxygen |
1390 |
| Sulfur |
29 |
| Total number of atoms |
14282 |
| Formula = C4505H7126N1232O1390S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
74440 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
73690 M-1 cm-1 |
| assuming all Cys residues are reduced |
73690 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
74440 M-1 cm-1 |
|
|
| Codon Bias |
0.041 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.426 |
| Hydropathicity of Protein |
-0.472 |
| Aromaticity Score |
0.080 |
|
