Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
66 |
4.3% |
| Arg[R] |
65 |
4.3% |
| Asn[N] |
89 |
5.8% |
| Asp[D] |
93 |
6.1% |
| Cys[C] |
6 |
0.4% |
| Gln[Q] |
63 |
4.1% |
| Glu[E] |
113 |
7.4% |
| Gly[G] |
82 |
5.4% |
| His[H] |
43 |
2.8% |
| Ile[I] |
95 |
6.2% |
| Leu[L] |
146 |
9.6% |
| Lys[K] |
138 |
9.0% |
| Met[M] |
36 |
2.4% |
| Phe[F] |
78 |
5.1% |
| Pro[P] |
91 |
6.0% |
| Ser[S] |
107 |
7.0% |
| Thr[T] |
73 |
4.8% |
| Trp[W] |
13 |
0.9% |
| Tyr[Y] |
59 |
3.9% |
| Val[V] |
72 |
4.7% |
| Total Length |
1528 |
|
| Instability index (II) |
42.86 |
| This classifies the protein as unstable |
|
| Carbon |
7894 |
| Hydrogen |
12308 |
| Nitrogen |
2112 |
| Oxygen |
2332 |
| Sulfur |
42 |
| Total number of atoms |
24688 |
| Formula = C7894H12308N2112O2332S42 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
159785 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
159410 M-1 cm-1 |
| assuming all Cys residues are reduced |
159410 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
159785 M-1 cm-1 |
|
|
| Codon Bias |
0.137 |
| Codon Adaptation Index |
0.194 |
| Frequency of Optimal Codons |
0.492 |
| Hydropathicity of Protein |
-0.602 |
| Aromaticity Score |
0.098 |
|
