Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
7.7% |
| Arg[R] |
21 |
4.9% |
| Asn[N] |
21 |
4.9% |
| Asp[D] |
24 |
5.6% |
| Cys[C] |
5 |
1.2% |
| Gln[Q] |
7 |
1.6% |
| Glu[E] |
26 |
6.1% |
| Gly[G] |
25 |
5.9% |
| His[H] |
9 |
2.1% |
| Ile[I] |
33 |
7.7% |
| Leu[L] |
47 |
11.0% |
| Lys[K] |
28 |
6.6% |
| Met[M] |
5 |
1.2% |
| Phe[F] |
14 |
3.3% |
| Pro[P] |
29 |
6.8% |
| Ser[S] |
35 |
8.2% |
| Thr[T] |
27 |
6.3% |
| Trp[W] |
2 |
0.5% |
| Tyr[Y] |
8 |
1.9% |
| Val[V] |
27 |
6.3% |
| Total Length |
426 |
|
| Instability index (II) |
37.27 |
| This classifies the protein as stable |
|
| Carbon |
2075 |
| Hydrogen |
3355 |
| Nitrogen |
565 |
| Oxygen |
625 |
| Sulfur |
10 |
| Total number of atoms |
6630 |
| Formula = C2075H3355N565O625S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
23170 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
22920 M-1 cm-1 |
| assuming all Cys residues are reduced |
22920 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
23170 M-1 cm-1 |
|
|
| Codon Bias |
0.013 |
| Codon Adaptation Index |
0.113 |
| Frequency of Optimal Codons |
0.401 |
| Hydropathicity of Protein |
-0.141 |
| Aromaticity Score |
0.056 |
|
