Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
6.3% |
| Arg[R] |
35 |
3.7% |
| Asn[N] |
58 |
6.1% |
| Asp[D] |
57 |
6.0% |
| Cys[C] |
14 |
1.5% |
| Gln[Q] |
22 |
2.3% |
| Glu[E] |
58 |
6.1% |
| Gly[G] |
55 |
5.8% |
| His[H] |
13 |
1.4% |
| Ile[I] |
74 |
7.8% |
| Leu[L] |
105 |
11.1% |
| Lys[K] |
63 |
6.6% |
| Met[M] |
31 |
3.3% |
| Phe[F] |
43 |
4.5% |
| Pro[P] |
35 |
3.7% |
| Ser[S] |
75 |
7.9% |
| Thr[T] |
66 |
6.9% |
| Trp[W] |
4 |
0.4% |
| Tyr[Y] |
12 |
1.3% |
| Val[V] |
70 |
7.4% |
| Total Length |
950 |
|
| Instability index (II) |
40.41 |
| This classifies the protein as unstable |
|
| Carbon |
4640 |
| Hydrogen |
7514 |
| Nitrogen |
1228 |
| Oxygen |
1414 |
| Sulfur |
45 |
| Total number of atoms |
14841 |
| Formula = C4640H7514N1228O1414S45 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
40755 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
39880 M-1 cm-1 |
| assuming all Cys residues are reduced |
39880 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
40755 M-1 cm-1 |
|
|
| Codon Bias |
0.171 |
| Codon Adaptation Index |
0.196 |
| Frequency of Optimal Codons |
0.509 |
| Hydropathicity of Protein |
0.019 |
| Aromaticity Score |
0.062 |
|
