Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
53 |
5.9% |
| Arg[R] |
61 |
6.8% |
| Asn[N] |
55 |
6.1% |
| Asp[D] |
58 |
6.5% |
| Cys[C] |
17 |
1.9% |
| Gln[Q] |
38 |
4.2% |
| Glu[E] |
53 |
5.9% |
| Gly[G] |
51 |
5.7% |
| His[H] |
21 |
2.3% |
| Ile[I] |
57 |
6.3% |
| Leu[L] |
90 |
10.0% |
| Lys[K] |
73 |
8.1% |
| Met[M] |
19 |
2.1% |
| Phe[F] |
28 |
3.1% |
| Pro[P] |
44 |
4.9% |
| Ser[S] |
60 |
6.7% |
| Thr[T] |
46 |
5.1% |
| Trp[W] |
7 |
0.8% |
| Tyr[Y] |
24 |
2.7% |
| Val[V] |
43 |
4.8% |
| Total Length |
898 |
|
| Instability index (II) |
40.19 |
| This classifies the protein as unstable |
|
| Carbon |
4470 |
| Hydrogen |
7198 |
| Nitrogen |
1296 |
| Oxygen |
1344 |
| Sulfur |
36 |
| Total number of atoms |
14344 |
| Formula = C4470H7198N1296O1344S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
75260 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
74260 M-1 cm-1 |
| assuming all Cys residues are reduced |
74260 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
75260 M-1 cm-1 |
|
|
| Codon Bias |
0.124 |
| Codon Adaptation Index |
0.155 |
| Frequency of Optimal Codons |
0.474 |
| Hydropathicity of Protein |
-0.576 |
| Aromaticity Score |
0.066 |
|
