Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
4.3% |
| Arg[R] |
48 |
4.4% |
| Asn[N] |
57 |
5.3% |
| Asp[D] |
70 |
6.5% |
| Cys[C] |
17 |
1.6% |
| Gln[Q] |
44 |
4.1% |
| Glu[E] |
65 |
6.0% |
| Gly[G] |
65 |
6.0% |
| His[H] |
27 |
2.5% |
| Ile[I] |
63 |
5.8% |
| Leu[L] |
87 |
8.0% |
| Lys[K] |
84 |
7.8% |
| Met[M] |
19 |
1.8% |
| Phe[F] |
59 |
5.4% |
| Pro[P] |
47 |
4.3% |
| Ser[S] |
89 |
8.2% |
| Thr[T] |
59 |
5.4% |
| Trp[W] |
26 |
2.4% |
| Tyr[Y] |
46 |
4.2% |
| Val[V] |
64 |
5.9% |
| Total Length |
1083 |
|
| Instability index (II) |
34.69 |
| This classifies the protein as stable |
|
| Carbon |
5613 |
| Hydrogen |
8578 |
| Nitrogen |
1492 |
| Oxygen |
1649 |
| Sulfur |
36 |
| Total number of atoms |
17368 |
| Formula = C5613H8578N1492O1649S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
212540 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
211540 M-1 cm-1 |
| assuming all Cys residues are reduced |
211540 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
212540 M-1 cm-1 |
|
|
| Codon Bias |
0.097 |
| Codon Adaptation Index |
0.176 |
| Frequency of Optimal Codons |
0.471 |
| Hydropathicity of Protein |
-0.500 |
| Aromaticity Score |
0.121 |
|
