NUT1/YGL151W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 49 4.3%
Arg[R] 28 2.5%
Asn[N] 96 8.5%
Asp[D] 70 6.2%
Cys[C] 11 1.0%
Gln[Q] 39 3.4%
Glu[E] 77 6.8%
Gly[G] 30 2.7%
His[H] 19 1.7%
Ile[I] 85 7.5%
Leu[L] 145 12.8%
Lys[K] 78 6.9%
Met[M] 15 1.3%
Phe[F] 64 5.7%
Pro[P] 38 3.4%
Ser[S] 109 9.6%
Thr[T] 70 6.2%
Trp[W] 7 0.6%
Tyr[Y] 38 3.4%
Val[V] 64 5.7%
Total Length 1132

Instability index
Instability index (II) 36.60
This classifies the protein as stable

Atomic composition
Carbon 5801
Hydrogen 9104
Nitrogen 1474
Oxygen 1779
Sulfur 26
Total number of atoms 18184
Formula = C5801H9104N1474O1779S26

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 95745 M-1 cm-1
assuming NO Cys residues appear as half cystines 95120 M-1 cm-1
assuming all Cys residues are reduced 95120 M-1 cm-1
assuming all pairs of Cys residues form cystines 95745 M-1 cm-1

Aliphatic index
Aliphatic index 99.96

Coding Region Translation Calculations
Codon Bias 0.022
Codon Adaptation Index 0.145
Frequency of Optimal Codons 0.431
Hydropathicity of Protein -0.192
Aromaticity Score 0.096