SCS3/YGL126W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 39 10.3%
Arg[R] 28 7.4%
Asn[N] 9 2.4%
Asp[D] 13 3.4%
Cys[C] 10 2.6%
Gln[Q] 17 4.5%
Glu[E] 5 1.3%
Gly[G] 33 8.7%
His[H] 19 5.0%
Ile[I] 17 4.5%
Leu[L] 46 12.1%
Lys[K] 8 2.1%
Met[M] 9 2.4%
Phe[F] 15 3.9%
Pro[P] 12 3.2%
Ser[S] 13 3.4%
Thr[T] 28 7.4%
Trp[W] 17 4.5%
Tyr[Y] 11 2.9%
Val[V] 31 8.2%
Total Length 380

Instability index
Instability index (II) 40.90
This classifies the protein as unstable

Atomic composition
Carbon 1951
Hydrogen 3003
Nitrogen 553
Oxygen 495
Sulfur 19
Total number of atoms 6021
Formula = C1951H3003N553O495S19

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 110515 M-1 cm-1
assuming NO Cys residues appear as half cystines 109890 M-1 cm-1
assuming all Cys residues are reduced 109890 M-1 cm-1
assuming all pairs of Cys residues form cystines 110515 M-1 cm-1

Aliphatic index
Aliphatic index 98.58

Coding Region Translation Calculations
Codon Bias 0.014
Codon Adaptation Index 0.066
Frequency of Optimal Codons 0.381
Hydropathicity of Protein 0.189
Aromaticity Score 0.113