Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
49 |
6.8% |
| Arg[R] |
32 |
4.4% |
| Asn[N] |
29 |
4.0% |
| Asp[D] |
28 |
3.9% |
| Cys[C] |
11 |
1.5% |
| Gln[Q] |
27 |
3.7% |
| Glu[E] |
29 |
4.0% |
| Gly[G] |
54 |
7.4% |
| His[H] |
7 |
1.0% |
| Ile[I] |
70 |
9.7% |
| Leu[L] |
78 |
10.8% |
| Lys[K] |
22 |
3.0% |
| Met[M] |
17 |
2.3% |
| Phe[F] |
39 |
5.4% |
| Pro[P] |
35 |
4.8% |
| Ser[S] |
67 |
9.2% |
| Thr[T] |
32 |
4.4% |
| Trp[W] |
15 |
2.1% |
| Tyr[Y] |
23 |
3.2% |
| Val[V] |
61 |
8.4% |
| Total Length |
725 |
|
| Instability index (II) |
45.12 |
| This classifies the protein as unstable |
|
| Carbon |
3667 |
| Hydrogen |
5736 |
| Nitrogen |
928 |
| Oxygen |
1018 |
| Sulfur |
28 |
| Total number of atoms |
11377 |
| Formula = C3667H5736N928O1018S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
117395 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
116770 M-1 cm-1 |
| assuming all Cys residues are reduced |
116770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
117395 M-1 cm-1 |
|
|
| Codon Bias |
0.027 |
| Codon Adaptation Index |
0.098 |
| Frequency of Optimal Codons |
0.414 |
| Hydropathicity of Protein |
0.386 |
| Aromaticity Score |
0.106 |
|
