SEH1/YGL100W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 25 7.2%
Arg[R] 15 4.3%
Asn[N] 13 3.7%
Asp[D] 30 8.6%
Cys[C] 8 2.3%
Gln[Q] 12 3.4%
Glu[E] 20 5.7%
Gly[G] 19 5.4%
His[H] 11 3.2%
Ile[I] 18 5.2%
Leu[L] 35 10.0%
Lys[K] 22 6.3%
Met[M] 5 1.4%
Phe[F] 9 2.6%
Pro[P] 12 3.4%
Ser[S] 43 12.3%
Thr[T] 14 4.0%
Trp[W] 12 3.4%
Tyr[Y] 9 2.6%
Val[V] 17 4.9%
Total Length 349

Instability index
Instability index (II) 54.10
This classifies the protein as unstable

Atomic composition
Carbon 1724
Hydrogen 2669
Nitrogen 475
Oxygen 541
Sulfur 13
Total number of atoms 5422
Formula = C1724H2669N475O541S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming all Cys residues are reduced 79410 M-1 cm-1
assuming all pairs of Cys residues form cystines 79910 M-1 cm-1

Aliphatic index
Aliphatic index 80.52

Coding Region Translation Calculations
Codon Bias 0.019
Codon Adaptation Index 0.143
Frequency of Optimal Codons 0.407
Hydropathicity of Protein -0.457
Aromaticity Score 0.086