TOS8/YGL096W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 10 3.6%
Arg[R] 18 6.5%
Asn[N] 31 11.2%
Asp[D] 1 0.4%
Cys[C] 0 0.0%
Gln[Q] 15 5.4%
Glu[E] 16 5.8%
Gly[G] 15 5.4%
His[H] 8 2.9%
Ile[I] 21 7.6%
Leu[L] 15 5.4%
Lys[K] 16 5.8%
Met[M] 5 1.8%
Phe[F] 11 4.0%
Pro[P] 24 8.7%
Ser[S] 27 9.8%
Thr[T] 15 5.4%
Trp[W] 2 0.7%
Tyr[Y] 9 3.3%
Val[V] 17 6.2%
Total Length 276

Instability index
Instability index (II) 56.64
This classifies the protein as unstable

Atomic composition
Carbon 1384
Hydrogen 2186
Nitrogen 410
Oxygen 408
Sulfur 5
Total number of atoms 4393
Formula = C1384H2186N410O408S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours


Aliphatic index
Aliphatic index 72.36

Coding Region Translation Calculations
Codon Bias 0.030
Codon Adaptation Index 0.103
Frequency of Optimal Codons 0.428
Hydropathicity of Protein -0.718
Aromaticity Score 0.080