MMS2/YGL087C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 4 2.9%
Arg[R] 7 5.1%
Asn[N] 8 5.8%
Asp[D] 8 5.8%
Cys[C] 3 2.2%
Gln[Q] 2 1.5%
Glu[E] 11 8.0%
Gly[G] 9 6.6%
His[H] 3 2.2%
Ile[I] 6 4.4%
Leu[L] 13 9.5%
Lys[K] 11 8.0%
Met[M] 4 2.9%
Phe[F] 5 3.6%
Pro[P] 12 8.8%
Ser[S] 9 6.6%
Thr[T] 12 8.8%
Trp[W] 2 1.5%
Tyr[Y] 4 2.9%
Val[V] 4 2.9%
Total Length 137

Instability index
Instability index (II) 36.56
This classifies the protein as stable

Atomic composition
Carbon 686
Hydrogen 1077
Nitrogen 187
Oxygen 211
Sulfur 7
Total number of atoms 2168
Formula = C686H1077N187O211S7

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming all Cys residues are reduced 16960 M-1 cm-1
assuming all pairs of Cys residues form cystines 17085 M-1 cm-1

Aliphatic index
Aliphatic index 65.47

Coding Region Translation Calculations
Codon Bias 0.240
Codon Adaptation Index 0.223
Frequency of Optimal Codons 0.542
Hydropathicity of Protein -0.740
Aromaticity Score 0.080