DBP3/YGL078C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 33 6.3%
Arg[R] 20 3.8%
Asn[N] 20 3.8%
Asp[D] 32 6.1%
Cys[C] 3 0.6%
Gln[Q] 23 4.4%
Glu[E] 44 8.4%
Gly[G] 29 5.5%
His[H] 11 2.1%
Ile[I] 27 5.2%
Leu[L] 49 9.4%
Lys[K] 69 13.2%
Met[M] 6 1.1%
Phe[F] 17 3.3%
Pro[P] 22 4.2%
Ser[S] 30 5.7%
Thr[T] 31 5.9%
Trp[W] 2 0.4%
Tyr[Y] 13 2.5%
Val[V] 42 8.0%
Total Length 523

Instability index
Instability index (II) 27.04
This classifies the protein as stable

Atomic composition
Carbon 2621
Hydrogen 4265
Nitrogen 719
Oxygen 793
Sulfur 9
Total number of atoms 8407
Formula = C2621H4265N719O793S9

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming all Cys residues are reduced 30370 M-1 cm-1
assuming all pairs of Cys residues form cystines 30495 M-1 cm-1

Aliphatic index
Aliphatic index 86.27

Coding Region Translation Calculations
Codon Bias 0.311
Codon Adaptation Index 0.271
Frequency of Optimal Codons 0.596
Hydropathicity of Protein -0.597
Aromaticity Score 0.061