Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
5.2% |
| Arg[R] |
31 |
4.7% |
| Asn[N] |
62 |
9.4% |
| Asp[D] |
41 |
6.2% |
| Cys[C] |
7 |
1.1% |
| Gln[Q] |
73 |
11.1% |
| Glu[E] |
29 |
4.4% |
| Gly[G] |
44 |
6.7% |
| His[H] |
18 |
2.7% |
| Ile[I] |
24 |
3.7% |
| Leu[L] |
38 |
5.8% |
| Lys[K] |
50 |
7.6% |
| Met[M] |
12 |
1.8% |
| Phe[F] |
12 |
1.8% |
| Pro[P] |
31 |
4.7% |
| Ser[S] |
71 |
10.8% |
| Thr[T] |
37 |
5.6% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
15 |
2.3% |
| Val[V] |
27 |
4.1% |
| Total Length |
657 |
|
| Instability index (II) |
46.87 |
| This classifies the protein as unstable |
|
| Carbon |
3064 |
| Hydrogen |
4918 |
| Nitrogen |
972 |
| Oxygen |
1056 |
| Sulfur |
19 |
| Total number of atoms |
10029 |
| Formula = C3064H4918N972O1056S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming all Cys residues are reduced |
27850 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
28225 M-1 cm-1 |
|
|
| Codon Bias |
0.055 |
| Codon Adaptation Index |
0.132 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-1.186 |
| Aromaticity Score |
0.043 |
|
