PYC1/YGL062W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 102 8.7%
Arg[R] 66 5.6%
Asn[N] 46 3.9%
Asp[D] 77 6.5%
Cys[C] 12 1.0%
Gln[Q] 50 4.2%
Glu[E] 75 6.4%
Gly[G] 90 7.6%
His[H] 29 2.5%
Ile[I] 72 6.1%
Leu[L] 104 8.8%
Lys[K] 65 5.5%
Met[M] 26 2.2%
Phe[F] 47 4.0%
Pro[P] 55 4.7%
Ser[S] 60 5.1%
Thr[T] 63 5.3%
Trp[W] 8 0.7%
Tyr[Y] 31 2.6%
Val[V] 100 8.5%
Total Length 1178

Instability index
Instability index (II) 33.82
This classifies the protein as stable

Atomic composition
Carbon 5782
Hydrogen 9181
Nitrogen 1603
Oxygen 1733
Sulfur 38
Total number of atoms 18337
Formula = C5782H9181N1603O1733S38

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming all Cys residues are reduced 90190 M-1 cm-1
assuming all pairs of Cys residues form cystines 90940 M-1 cm-1

Aliphatic index
Aliphatic index 91.54

Coding Region Translation Calculations
Codon Bias 0.306
Codon Adaptation Index 0.261
Frequency of Optimal Codons 0.582
Hydropathicity of Protein -0.205
Aromaticity Score 0.073