RNA15/YGL044C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 17 5.7%
Arg[R] 10 3.4%
Asn[N] 35 11.8%
Asp[D] 15 5.1%
Cys[C] 4 1.4%
Gln[Q] 24 8.1%
Glu[E] 17 5.7%
Gly[G] 18 6.1%
His[H] 1 0.3%
Ile[I] 14 4.7%
Leu[L] 30 10.1%
Lys[K] 15 5.1%
Met[M] 7 2.4%
Phe[F] 12 4.1%
Pro[P] 16 5.4%
Ser[S] 27 9.1%
Thr[T] 8 2.7%
Trp[W] 2 0.7%
Tyr[Y] 6 2.0%
Val[V] 18 6.1%
Total Length 296

Instability index
Instability index (II) 41.31
This classifies the protein as unstable

Atomic composition
Carbon 1426
Hydrogen 2258
Nitrogen 404
Oxygen 461
Sulfur 11
Total number of atoms 4560
Formula = C1426H2258N404O461S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming all Cys residues are reduced 19940 M-1 cm-1
assuming all pairs of Cys residues form cystines 20190 M-1 cm-1

Aliphatic index
Aliphatic index 81.35

Coding Region Translation Calculations
Codon Bias -0.036
Codon Adaptation Index 0.097
Frequency of Optimal Codons 0.380
Hydropathicity of Protein -0.523
Aromaticity Score 0.068