Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
9.6% |
| Arg[R] |
11 |
2.8% |
| Asn[N] |
46 |
11.6% |
| Asp[D] |
15 |
3.8% |
| Cys[C] |
2 |
0.5% |
| Gln[Q] |
41 |
10.3% |
| Glu[E] |
11 |
2.8% |
| Gly[G] |
20 |
5.0% |
| His[H] |
8 |
2.0% |
| Ile[I] |
15 |
3.8% |
| Leu[L] |
33 |
8.3% |
| Lys[K] |
18 |
4.5% |
| Met[M] |
20 |
5.0% |
| Phe[F] |
8 |
2.0% |
| Pro[P] |
28 |
7.1% |
| Ser[S] |
32 |
8.1% |
| Thr[T] |
32 |
8.1% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
6 |
1.5% |
| Val[V] |
13 |
3.3% |
| Total Length |
397 |
|
| Instability index (II) |
39.79 |
| This classifies the protein as stable |
|
| Carbon |
1829 |
| Hydrogen |
2953 |
| Nitrogen |
551 |
| Oxygen |
607 |
| Sulfur |
22 |
| Total number of atoms |
5962 |
| Formula = C1829H2953N551O607S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
9065 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
8940 M-1 cm-1 |
| assuming all Cys residues are reduced |
8940 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
9065 M-1 cm-1 |
|
|
| Codon Bias |
0.081 |
| Codon Adaptation Index |
0.105 |
| Frequency of Optimal Codons |
0.446 |
| Hydropathicity of Protein |
-0.675 |
| Aromaticity Score |
0.035 |
|
