Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
51 |
5.7% |
| Arg[R] |
21 |
2.4% |
| Asn[N] |
90 |
10.1% |
| Asp[D] |
48 |
5.4% |
| Cys[C] |
11 |
1.2% |
| Gln[Q] |
43 |
4.8% |
| Glu[E] |
38 |
4.3% |
| Gly[G] |
45 |
5.1% |
| His[H] |
29 |
3.3% |
| Ile[I] |
52 |
5.9% |
| Leu[L] |
76 |
8.6% |
| Lys[K] |
52 |
5.9% |
| Met[M] |
26 |
2.9% |
| Phe[F] |
32 |
3.6% |
| Pro[P] |
76 |
8.6% |
| Ser[S] |
86 |
9.7% |
| Thr[T] |
49 |
5.5% |
| Trp[W] |
2 |
0.2% |
| Tyr[Y] |
23 |
2.6% |
| Val[V] |
38 |
4.3% |
| Total Length |
888 |
|
| Instability index (II) |
50.92 |
| This classifies the protein as unstable |
|
| Carbon |
4284 |
| Hydrogen |
6720 |
| Nitrogen |
1196 |
| Oxygen |
1352 |
| Sulfur |
37 |
| Total number of atoms |
13589 |
| Formula = C4284H6720N1196O1352S37 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
45895 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
45270 M-1 cm-1 |
| assuming all Cys residues are reduced |
45270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
45895 M-1 cm-1 |
|
|
| Codon Bias |
0.065 |
| Codon Adaptation Index |
0.152 |
| Frequency of Optimal Codons |
0.444 |
| Hydropathicity of Protein |
-0.552 |
| Aromaticity Score |
0.064 |
|
