LEU1/YGL009C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 61 7.8%
Arg[R] 33 4.2%
Asn[N] 29 3.7%
Asp[D] 55 7.1%
Cys[C] 11 1.4%
Gln[Q] 26 3.3%
Glu[E] 55 7.1%
Gly[G] 63 8.1%
His[H] 17 2.2%
Ile[I] 55 7.1%
Leu[L] 58 7.4%
Lys[K] 60 7.7%
Met[M] 15 1.9%
Phe[F] 33 4.2%
Pro[P] 40 5.1%
Ser[S] 47 6.0%
Thr[T] 48 6.2%
Trp[W] 8 1.0%
Tyr[Y] 17 2.2%
Val[V] 48 6.2%
Total Length 779

Instability index
Instability index (II) 32.92
This classifies the protein as stable

Atomic composition
Carbon 3807
Hydrogen 6019
Nitrogen 1035
Oxygen 1167
Sulfur 26
Total number of atoms 12054
Formula = C3807H6019N1035O1167S26

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 69955 M-1 cm-1
assuming NO Cys residues appear as half cystines 69330 M-1 cm-1
assuming all Cys residues are reduced 69330 M-1 cm-1
assuming all pairs of Cys residues form cystines 69955 M-1 cm-1

Aliphatic index
Aliphatic index 82.27

Coding Region Translation Calculations
Codon Bias 0.364
Codon Adaptation Index 0.336
Frequency of Optimal Codons 0.623
Hydropathicity of Protein -0.355
Aromaticity Score 0.074