Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
61 |
7.8% |
| Arg[R] |
33 |
4.2% |
| Asn[N] |
29 |
3.7% |
| Asp[D] |
55 |
7.1% |
| Cys[C] |
11 |
1.4% |
| Gln[Q] |
26 |
3.3% |
| Glu[E] |
55 |
7.1% |
| Gly[G] |
63 |
8.1% |
| His[H] |
17 |
2.2% |
| Ile[I] |
55 |
7.1% |
| Leu[L] |
58 |
7.4% |
| Lys[K] |
60 |
7.7% |
| Met[M] |
15 |
1.9% |
| Phe[F] |
33 |
4.2% |
| Pro[P] |
40 |
5.1% |
| Ser[S] |
47 |
6.0% |
| Thr[T] |
48 |
6.2% |
| Trp[W] |
8 |
1.0% |
| Tyr[Y] |
17 |
2.2% |
| Val[V] |
48 |
6.2% |
| Total Length |
779 |
|
| Instability index (II) |
32.92 |
| This classifies the protein as stable |
|
| Carbon |
3807 |
| Hydrogen |
6019 |
| Nitrogen |
1035 |
| Oxygen |
1167 |
| Sulfur |
26 |
| Total number of atoms |
12054 |
| Formula = C3807H6019N1035O1167S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
69955 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
69330 M-1 cm-1 |
| assuming all Cys residues are reduced |
69330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
69955 M-1 cm-1 |
|
|
| Codon Bias |
0.364 |
| Codon Adaptation Index |
0.336 |
| Frequency of Optimal Codons |
0.623 |
| Hydropathicity of Protein |
-0.355 |
| Aromaticity Score |
0.074 |
|