BRP1/YGL007W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 10 8.0%
Arg[R] 10 8.0%
Asn[N] 3 2.4%
Asp[D] 4 3.2%
Cys[C] 2 1.6%
Gln[Q] 4 3.2%
Glu[E] 5 4.0%
Gly[G] 8 6.4%
His[H] 2 1.6%
Ile[I] 7 5.6%
Leu[L] 7 5.6%
Lys[K] 7 5.6%
Met[M] 8 6.4%
Phe[F] 6 4.8%
Pro[P] 8 6.4%
Ser[S] 15 12.0%
Thr[T] 3 2.4%
Trp[W] 1 0.8%
Tyr[Y] 2 1.6%
Val[V] 13 10.4%
Total Length 125

Instability index
Instability index (II) 68.92
This classifies the protein as unstable

Atomic composition
Carbon 608
Hydrogen 986
Nitrogen 174
Oxygen 171
Sulfur 10
Total number of atoms 1949
Formula = C608H986N174O171S10

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 8605 M-1 cm-1
assuming NO Cys residues appear as half cystines 8480 M-1 cm-1
assuming all Cys residues are reduced 8480 M-1 cm-1
assuming all pairs of Cys residues form cystines 8605 M-1 cm-1

Aliphatic index
Aliphatic index 81.84

Coding Region Translation Calculations
Codon Bias -0.119
Codon Adaptation Index 0.059
Frequency of Optimal Codons 0.310
Hydropathicity of Protein -0.005
Aromaticity Score 0.072