Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
76 |
6.5% |
| Arg[R] |
54 |
4.6% |
| Asn[N] |
73 |
6.2% |
| Asp[D] |
73 |
6.2% |
| Cys[C] |
17 |
1.4% |
| Gln[Q] |
43 |
3.7% |
| Glu[E] |
64 |
5.5% |
| Gly[G] |
66 |
5.6% |
| His[H] |
13 |
1.1% |
| Ile[I] |
80 |
6.8% |
| Leu[L] |
119 |
10.1% |
| Lys[K] |
83 |
7.1% |
| Met[M] |
28 |
2.4% |
| Phe[F] |
61 |
5.2% |
| Pro[P] |
39 |
3.3% |
| Ser[S] |
102 |
8.7% |
| Thr[T] |
62 |
5.3% |
| Trp[W] |
11 |
0.9% |
| Tyr[Y] |
25 |
2.1% |
| Val[V] |
84 |
7.2% |
| Total Length |
1173 |
|
| Instability index (II) |
36.03 |
| This classifies the protein as stable |
|
| Carbon |
5828 |
| Hydrogen |
9293 |
| Nitrogen |
1571 |
| Oxygen |
1753 |
| Sulfur |
45 |
| Total number of atoms |
18490 |
| Formula = C5828H9293N1571O1753S45 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
98750 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
97750 M-1 cm-1 |
| assuming all Cys residues are reduced |
97750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
98750 M-1 cm-1 |
|
|
| Codon Bias |
0.090 |
| Codon Adaptation Index |
0.159 |
| Frequency of Optimal Codons |
0.459 |
| Hydropathicity of Protein |
-0.155 |
| Aromaticity Score |
0.083 |
|
