FIT1/YDR534C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 78 14.8%
Arg[R] 7 1.3%
Asn[N] 10 1.9%
Asp[D] 9 1.7%
Cys[C] 0 0.0%
Gln[Q] 12 2.3%
Glu[E] 40 7.6%
Gly[G] 28 5.3%
His[H] 5 0.9%
Ile[I] 24 4.5%
Leu[L] 8 1.5%
Lys[K] 14 2.7%
Met[M] 3 0.6%
Phe[F] 8 1.5%
Pro[P] 13 2.5%
Ser[S] 105 19.9%
Thr[T] 103 19.5%
Trp[W] 5 0.9%
Tyr[Y] 6 1.1%
Val[V] 50 9.5%
Total Length 528

Instability index
Instability index (II) 31.55
This classifies the protein as stable

Atomic composition
Carbon 2212
Hydrogen 3586
Nitrogen 600
Oxygen 863
Sulfur 3
Total number of atoms 7264
Formula = C2212H3586N600O863S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 36440 M-1 cm-1

Aliphatic index
Aliphatic index 65.87

Coding Region Translation Calculations
Codon Bias 0.406
Codon Adaptation Index 0.259
Frequency of Optimal Codons 0.648
Hydropathicity of Protein -0.065
Aromaticity Score 0.036