Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
78 |
14.8% |
| Arg[R] |
7 |
1.3% |
| Asn[N] |
10 |
1.9% |
| Asp[D] |
9 |
1.7% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
12 |
2.3% |
| Glu[E] |
40 |
7.6% |
| Gly[G] |
28 |
5.3% |
| His[H] |
5 |
0.9% |
| Ile[I] |
24 |
4.5% |
| Leu[L] |
8 |
1.5% |
| Lys[K] |
14 |
2.7% |
| Met[M] |
3 |
0.6% |
| Phe[F] |
8 |
1.5% |
| Pro[P] |
13 |
2.5% |
| Ser[S] |
105 |
19.9% |
| Thr[T] |
103 |
19.5% |
| Trp[W] |
5 |
0.9% |
| Tyr[Y] |
6 |
1.1% |
| Val[V] |
50 |
9.5% |
| Total Length |
528 |
|
| Instability index (II) |
31.55 |
| This classifies the protein as stable |
|
| Carbon |
2212 |
| Hydrogen |
3586 |
| Nitrogen |
600 |
| Oxygen |
863 |
| Sulfur |
3 |
| Total number of atoms |
7264 |
| Formula = C2212H3586N600O863S3 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
36440 M-1 cm-1 |
|
|
| Codon Bias |
0.406 |
| Codon Adaptation Index |
0.259 |
| Frequency of Optimal Codons |
0.648 |
| Hydropathicity of Protein |
-0.065 |
| Aromaticity Score |
0.036 |
|