GIN4/YDR507C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 49 4.3%
Arg[R] 75 6.6%
Asn[N] 66 5.8%
Asp[D] 57 5.0%
Cys[C] 4 0.4%
Gln[Q] 42 3.7%
Glu[E] 112 9.8%
Gly[G] 54 4.7%
His[H] 19 1.7%
Ile[I] 74 6.5%
Leu[L] 108 9.5%
Lys[K] 118 10.3%
Met[M] 15 1.3%
Phe[F] 35 3.1%
Pro[P] 50 4.4%
Ser[S] 119 10.4%
Thr[T] 61 5.3%
Trp[W] 6 0.5%
Tyr[Y] 24 2.1%
Val[V] 54 4.7%
Total Length 1142

Instability index
Instability index (II) 53.41
This classifies the protein as unstable

Atomic composition
Carbon 5686
Hydrogen 9265
Nitrogen 1637
Oxygen 1793
Sulfur 19
Total number of atoms 18400
Formula = C5686H9265N1637O1793S19

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 69010 M-1 cm-1
assuming NO Cys residues appear as half cystines 68760 M-1 cm-1
assuming all Cys residues are reduced 68760 M-1 cm-1
assuming all pairs of Cys residues form cystines 69010 M-1 cm-1

Aliphatic index
Aliphatic index 80.16

Coding Region Translation Calculations
Codon Bias 0.104
Codon Adaptation Index 0.158
Frequency of Optimal Codons 0.467
Hydropathicity of Protein -0.796
Aromaticity Score 0.057