CWC21/YDR482C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 6.7%
Arg[R] 15 11.1%
Asn[N] 5 3.7%
Asp[D] 3 2.2%
Cys[C] 1 0.7%
Gln[Q] 16 11.9%
Glu[E] 17 12.6%
Gly[G] 5 3.7%
His[H] 5 3.7%
Ile[I] 5 3.7%
Leu[L] 10 7.4%
Lys[K] 11 8.1%
Met[M] 3 2.2%
Phe[F] 0 0.0%
Pro[P] 3 2.2%
Ser[S] 14 10.4%
Thr[T] 6 4.4%
Trp[W] 1 0.7%
Tyr[Y] 2 1.5%
Val[V] 4 3.0%
Total Length 135

Instability index
Instability index (II) 75.12
This classifies the protein as unstable

Atomic composition
Carbon 658
Hydrogen 1095
Nitrogen 223
Oxygen 219
Sulfur 4
Total number of atoms 2199
Formula = C658H1095N223O219S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 8480 M-1 cm-1
assuming NO Cys residues appear as half cystines 8480 M-1 cm-1
assuming all Cys residues are reduced 8480 M-1 cm-1
assuming all pairs of Cys residues form cystines 8480 M-1 cm-1

Aliphatic index
Aliphatic index 58.59

Coding Region Translation Calculations
Codon Bias -0.081
Codon Adaptation Index 0.056
Frequency of Optimal Codons 0.344
Hydropathicity of Protein -1.436
Aromaticity Score 0.022