SNF1/YDR477W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 33 5.2%
Arg[R] 22 3.5%
Asn[N] 40 6.3%
Asp[D] 41 6.5%
Cys[C] 4 0.6%
Gln[Q] 24 3.8%
Glu[E] 42 6.6%
Gly[G] 29 4.6%
His[H] 31 4.9%
Ile[I] 47 7.4%
Leu[L] 55 8.7%
Lys[K] 48 7.6%
Met[M] 19 3.0%
Phe[F] 20 3.2%
Pro[P] 30 4.7%
Ser[S] 59 9.3%
Thr[T] 30 4.7%
Trp[W] 7 1.1%
Tyr[Y] 23 3.6%
Val[V] 29 4.6%
Total Length 633

Instability index
Instability index (II) 52.27
This classifies the protein as unstable

Atomic composition
Carbon 3192
Hydrogen 4988
Nitrogen 880
Oxygen 976
Sulfur 23
Total number of atoms 10059
Formula = C3192H4988N880O976S23

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 73020 M-1 cm-1
assuming NO Cys residues appear as half cystines 72770 M-1 cm-1
assuming all Cys residues are reduced 72770 M-1 cm-1
assuming all pairs of Cys residues form cystines 73020 M-1 cm-1

Aliphatic index
Aliphatic index 81.34

Coding Region Translation Calculations
Codon Bias 0.119
Codon Adaptation Index 0.188
Frequency of Optimal Codons 0.486
Hydropathicity of Protein -0.569
Aromaticity Score 0.079