Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
100 |
7.0% |
| Arg[R] |
71 |
4.9% |
| Asn[N] |
91 |
6.3% |
| Asp[D] |
87 |
6.1% |
| Cys[C] |
3 |
0.2% |
| Gln[Q] |
62 |
4.3% |
| Glu[E] |
149 |
10.4% |
| Gly[G] |
36 |
2.5% |
| His[H] |
32 |
2.2% |
| Ile[I] |
74 |
5.2% |
| Leu[L] |
100 |
7.0% |
| Lys[K] |
183 |
12.8% |
| Met[M] |
15 |
1.0% |
| Phe[F] |
23 |
1.6% |
| Pro[P] |
84 |
5.9% |
| Ser[S] |
138 |
9.6% |
| Thr[T] |
93 |
6.5% |
| Trp[W] |
8 |
0.6% |
| Tyr[Y] |
15 |
1.0% |
| Val[V] |
71 |
4.9% |
| Total Length |
1435 |
|
| Instability index (II) |
54.06 |
| This classifies the protein as unstable |
|
| Carbon |
6974 |
| Hydrogen |
11456 |
| Nitrogen |
2056 |
| Oxygen |
2307 |
| Sulfur |
18 |
| Total number of atoms |
22811 |
| Formula = C6974H11456N2056O2307S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
66475 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
66350 M-1 cm-1 |
| assuming all Cys residues are reduced |
66350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
66475 M-1 cm-1 |
|
|
| Codon Bias |
0.051 |
| Codon Adaptation Index |
0.141 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-1.085 |
| Aromaticity Score |
0.032 |
|
