Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
37 |
5.8% |
| Arg[R] |
17 |
2.7% |
| Asn[N] |
35 |
5.5% |
| Asp[D] |
33 |
5.2% |
| Cys[C] |
7 |
1.1% |
| Gln[Q] |
19 |
3.0% |
| Glu[E] |
26 |
4.1% |
| Gly[G] |
38 |
6.0% |
| His[H] |
8 |
1.3% |
| Ile[I] |
63 |
10.0% |
| Leu[L] |
76 |
12.0% |
| Lys[K] |
18 |
2.8% |
| Met[M] |
16 |
2.5% |
| Phe[F] |
41 |
6.5% |
| Pro[P] |
28 |
4.4% |
| Ser[S] |
60 |
9.5% |
| Thr[T] |
38 |
6.0% |
| Trp[W] |
7 |
1.1% |
| Tyr[Y] |
23 |
3.6% |
| Val[V] |
43 |
6.8% |
| Total Length |
633 |
|
| Instability index (II) |
41.99 |
| This classifies the protein as unstable |
|
| Carbon |
3217 |
| Hydrogen |
4989 |
| Nitrogen |
779 |
| Oxygen |
927 |
| Sulfur |
23 |
| Total number of atoms |
9935 |
| Formula = C3217H4989N779O927S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
73145 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
72770 M-1 cm-1 |
| assuming all Cys residues are reduced |
72770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
73145 M-1 cm-1 |
|
|
| Codon Bias |
0.139 |
| Codon Adaptation Index |
0.156 |
| Frequency of Optimal Codons |
0.490 |
| Hydropathicity of Protein |
0.385 |
| Aromaticity Score |
0.112 |
|
