Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
65 |
4.6% |
| Arg[R] |
38 |
2.7% |
| Asn[N] |
116 |
8.2% |
| Asp[D] |
95 |
6.7% |
| Cys[C] |
15 |
1.1% |
| Gln[Q] |
60 |
4.2% |
| Glu[E] |
82 |
5.8% |
| Gly[G] |
55 |
3.9% |
| His[H] |
35 |
2.5% |
| Ile[I] |
101 |
7.1% |
| Leu[L] |
141 |
9.9% |
| Lys[K] |
102 |
7.2% |
| Met[M] |
33 |
2.3% |
| Phe[F] |
61 |
4.3% |
| Pro[P] |
87 |
6.1% |
| Ser[S] |
137 |
9.6% |
| Thr[T] |
78 |
5.5% |
| Trp[W] |
12 |
0.8% |
| Tyr[Y] |
41 |
2.9% |
| Val[V] |
66 |
4.6% |
| Total Length |
1420 |
|
| Instability index (II) |
47.03 |
| This classifies the protein as unstable |
|
| Carbon |
7109 |
| Hydrogen |
11148 |
| Nitrogen |
1894 |
| Oxygen |
2207 |
| Sulfur |
48 |
| Total number of atoms |
22406 |
| Formula = C7109H11148N1894O2207S48 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
127965 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
127090 M-1 cm-1 |
| assuming all Cys residues are reduced |
127090 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
127965 M-1 cm-1 |
|
|
| Codon Bias |
0.019 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.425 |
| Hydropathicity of Protein |
-0.458 |
| Aromaticity Score |
0.080 |
|
