Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
5.4% |
| Arg[R] |
24 |
3.7% |
| Asn[N] |
39 |
6.1% |
| Asp[D] |
46 |
7.2% |
| Cys[C] |
7 |
1.1% |
| Gln[Q] |
26 |
4.0% |
| Glu[E] |
54 |
8.4% |
| Gly[G] |
34 |
5.3% |
| His[H] |
13 |
2.0% |
| Ile[I] |
36 |
5.6% |
| Leu[L] |
69 |
10.7% |
| Lys[K] |
41 |
6.4% |
| Met[M] |
8 |
1.2% |
| Phe[F] |
17 |
2.6% |
| Pro[P] |
17 |
2.6% |
| Ser[S] |
61 |
9.5% |
| Thr[T] |
41 |
6.4% |
| Trp[W] |
10 |
1.6% |
| Tyr[Y] |
18 |
2.8% |
| Val[V] |
47 |
7.3% |
| Total Length |
643 |
|
| Instability index (II) |
40.77 |
| This classifies the protein as unstable |
|
| Carbon |
3164 |
| Hydrogen |
5011 |
| Nitrogen |
857 |
| Oxygen |
1029 |
| Sulfur |
15 |
| Total number of atoms |
10076 |
| Formula = C3164H5011N857O1029S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
82195 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
81820 M-1 cm-1 |
| assuming all Cys residues are reduced |
81820 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
82195 M-1 cm-1 |
|
|
| Codon Bias |
0.139 |
| Codon Adaptation Index |
0.167 |
| Frequency of Optimal Codons |
0.491 |
| Hydropathicity of Protein |
-0.424 |
| Aromaticity Score |
0.070 |
|
