Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
40 |
7.2% |
| Arg[R] |
27 |
4.9% |
| Asn[N] |
21 |
3.8% |
| Asp[D] |
20 |
3.6% |
| Cys[C] |
10 |
1.8% |
| Gln[Q] |
11 |
2.0% |
| Glu[E] |
18 |
3.2% |
| Gly[G] |
32 |
5.8% |
| His[H] |
5 |
0.9% |
| Ile[I] |
53 |
9.5% |
| Leu[L] |
68 |
12.3% |
| Lys[K] |
15 |
2.7% |
| Met[M] |
17 |
3.1% |
| Phe[F] |
35 |
6.3% |
| Pro[P] |
21 |
3.8% |
| Ser[S] |
59 |
10.6% |
| Thr[T] |
39 |
7.0% |
| Trp[W] |
6 |
1.1% |
| Tyr[Y] |
23 |
4.1% |
| Val[V] |
35 |
6.3% |
| Total Length |
555 |
|
| Instability index (II) |
44.31 |
| This classifies the protein as unstable |
|
| Carbon |
2817 |
| Hydrogen |
4423 |
| Nitrogen |
699 |
| Oxygen |
785 |
| Sulfur |
27 |
| Total number of atoms |
8751 |
| Formula = C2817H4423N699O785S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
67895 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
67270 M-1 cm-1 |
| assuming all Cys residues are reduced |
67270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
67895 M-1 cm-1 |
|
|
| Codon Bias |
0.090 |
| Codon Adaptation Index |
0.123 |
| Frequency of Optimal Codons |
0.449 |
| Hydropathicity of Protein |
0.494 |
| Aromaticity Score |
0.115 |
|
