RPP2B/YDR382W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 23 20.9%
Arg[R] 1 0.9%
Asn[N] 2 1.8%
Asp[D] 7 6.4%
Cys[C] 0 0.0%
Gln[Q] 2 1.8%
Glu[E] 16 14.5%
Gly[G] 14 12.7%
His[H] 0 0.0%
Ile[I] 4 3.6%
Leu[L] 9 8.2%
Lys[K] 7 6.4%
Met[M] 2 1.8%
Phe[F] 3 2.7%
Pro[P] 2 1.8%
Ser[S] 8 7.3%
Thr[T] 2 1.8%
Trp[W] 0 0.0%
Tyr[Y] 2 1.8%
Val[V] 6 5.5%
Total Length 110

Instability index
Instability index (II) 36.33
This classifies the protein as stable

Atomic composition
Carbon 476
Hydrogen 758
Nitrogen 124
Oxygen 173
Sulfur 2
Total number of atoms 1533
Formula = C476H758N124O173S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 82.82

Coding Region Translation Calculations
Codon Bias 0.720
Codon Adaptation Index 0.762
Frequency of Optimal Codons 0.824
Hydropathicity of Protein -0.132
Aromaticity Score 0.045