ATP22/YDR350C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 26 3.8%
Arg[R] 31 4.5%
Asn[N] 46 6.7%
Asp[D] 21 3.1%
Cys[C] 13 1.9%
Gln[Q] 24 3.5%
Glu[E] 43 6.3%
Gly[G] 26 3.8%
His[H] 21 3.1%
Ile[I] 40 5.8%
Leu[L] 93 13.6%
Lys[K] 57 8.3%
Met[M] 14 2.0%
Phe[F] 45 6.6%
Pro[P] 24 3.5%
Ser[S] 52 7.6%
Thr[T] 38 5.6%
Trp[W] 7 1.0%
Tyr[Y] 31 4.5%
Val[V] 32 4.7%
Total Length 684

Instability index
Instability index (II) 46.36
This classifies the protein as unstable

Atomic composition
Carbon 3643
Hydrogen 5677
Nitrogen 953
Oxygen 1004
Sulfur 27
Total number of atoms 11304
Formula = C3643H5677N953O1004S27

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 79940 M-1 cm-1
assuming NO Cys residues appear as half cystines 79190 M-1 cm-1
assuming all Cys residues are reduced 84690 M-1 cm-1
assuming all pairs of Cys residues form cystines 85440 M-1 cm-1

Aliphatic index
Aliphatic index 93.20

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000