Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
7.9% |
| Arg[R] |
20 |
3.5% |
| Asn[N] |
18 |
3.2% |
| Asp[D] |
15 |
2.6% |
| Cys[C] |
11 |
1.9% |
| Gln[Q] |
13 |
2.3% |
| Glu[E] |
29 |
5.1% |
| Gly[G] |
53 |
9.3% |
| His[H] |
6 |
1.1% |
| Ile[I] |
47 |
8.2% |
| Leu[L] |
41 |
7.2% |
| Lys[K] |
26 |
4.6% |
| Met[M] |
20 |
3.5% |
| Phe[F] |
35 |
6.1% |
| Pro[P] |
22 |
3.9% |
| Ser[S] |
44 |
7.7% |
| Thr[T] |
33 |
5.8% |
| Trp[W] |
13 |
2.3% |
| Tyr[Y] |
25 |
4.4% |
| Val[V] |
54 |
9.5% |
| Total Length |
570 |
|
| Instability index (II) |
37.04 |
| This classifies the protein as stable |
|
| Carbon |
2883 |
| Hydrogen |
4434 |
| Nitrogen |
712 |
| Oxygen |
792 |
| Sulfur |
31 |
| Total number of atoms |
8852 |
| Formula = C2883H4434N712O792S31 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
109375 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
108750 M-1 cm-1 |
| assuming all Cys residues are reduced |
108750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
109375 M-1 cm-1 |
|
|
| Codon Bias |
0.620 |
| Codon Adaptation Index |
0.519 |
| Frequency of Optimal Codons |
0.775 |
| Hydropathicity of Protein |
0.362 |
| Aromaticity Score |
0.128 |
|
