Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
78 |
5.2% |
| Arg[R] |
85 |
5.6% |
| Asn[N] |
82 |
5.4% |
| Asp[D] |
120 |
7.9% |
| Cys[C] |
14 |
0.9% |
| Gln[Q] |
72 |
4.8% |
| Glu[E] |
132 |
8.7% |
| Gly[G] |
60 |
4.0% |
| His[H] |
41 |
2.7% |
| Ile[I] |
66 |
4.4% |
| Leu[L] |
158 |
10.4% |
| Lys[K] |
137 |
9.0% |
| Met[M] |
24 |
1.6% |
| Phe[F] |
63 |
4.2% |
| Pro[P] |
44 |
2.9% |
| Ser[S] |
115 |
7.6% |
| Thr[T] |
83 |
5.5% |
| Trp[W] |
10 |
0.7% |
| Tyr[Y] |
47 |
3.1% |
| Val[V] |
83 |
5.5% |
| Total Length |
1514 |
|
| Instability index (II) |
44.03 |
| This classifies the protein as unstable |
|
| Carbon |
7678 |
| Hydrogen |
12166 |
| Nitrogen |
2152 |
| Oxygen |
2418 |
| Sulfur |
38 |
| Total number of atoms |
24452 |
| Formula = C7678H12166N2152O2418S38 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
125905 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
125030 M-1 cm-1 |
| assuming all Cys residues are reduced |
125030 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
125905 M-1 cm-1 |
|
|
| Codon Bias |
0.056 |
| Codon Adaptation Index |
0.145 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.753 |
| Aromaticity Score |
0.079 |
|
