Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
5.8% |
| Arg[R] |
49 |
4.7% |
| Asn[N] |
81 |
7.8% |
| Asp[D] |
81 |
7.8% |
| Cys[C] |
15 |
1.4% |
| Gln[Q] |
36 |
3.5% |
| Glu[E] |
90 |
8.7% |
| Gly[G] |
28 |
2.7% |
| His[H] |
18 |
1.7% |
| Ile[I] |
92 |
8.9% |
| Leu[L] |
109 |
10.5% |
| Lys[K] |
63 |
6.1% |
| Met[M] |
18 |
1.7% |
| Phe[F] |
42 |
4.1% |
| Pro[P] |
24 |
2.3% |
| Ser[S] |
82 |
7.9% |
| Thr[T] |
52 |
5.0% |
| Trp[W] |
6 |
0.6% |
| Tyr[Y] |
23 |
2.2% |
| Val[V] |
66 |
6.4% |
| Total Length |
1035 |
|
| Instability index (II) |
47.09 |
| This classifies the protein as unstable |
|
| Carbon |
5190 |
| Hydrogen |
8294 |
| Nitrogen |
1404 |
| Oxygen |
1652 |
| Sulfur |
33 |
| Total number of atoms |
16573 |
| Formula = C5190H8294N1404O1652S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
68145 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
67270 M-1 cm-1 |
| assuming all Cys residues are reduced |
67270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
68145 M-1 cm-1 |
|
|
| Codon Bias |
0.030 |
| Codon Adaptation Index |
0.132 |
| Frequency of Optimal Codons |
0.439 |
| Hydropathicity of Protein |
-0.305 |
| Aromaticity Score |
0.069 |
|
