Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
25 |
3.2% |
| Arg[R] |
37 |
4.8% |
| Asn[N] |
62 |
8.0% |
| Asp[D] |
57 |
7.3% |
| Cys[C] |
13 |
1.7% |
| Gln[Q] |
25 |
3.2% |
| Glu[E] |
45 |
5.8% |
| Gly[G] |
38 |
4.9% |
| His[H] |
12 |
1.5% |
| Ile[I] |
56 |
7.2% |
| Leu[L] |
69 |
8.9% |
| Lys[K] |
59 |
7.6% |
| Met[M] |
12 |
1.5% |
| Phe[F] |
32 |
4.1% |
| Pro[P] |
20 |
2.6% |
| Ser[S] |
78 |
10.1% |
| Thr[T] |
49 |
6.3% |
| Trp[W] |
17 |
2.2% |
| Tyr[Y] |
10 |
1.3% |
| Val[V] |
60 |
7.7% |
| Total Length |
776 |
|
| Instability index (II) |
35.10 |
| This classifies the protein as stable |
|
| Carbon |
3869 |
| Hydrogen |
6158 |
| Nitrogen |
1074 |
| Oxygen |
1205 |
| Sulfur |
25 |
| Total number of atoms |
12331 |
| Formula = C3869H6158N1074O1205S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
109150 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
108400 M-1 cm-1 |
| assuming all Cys residues are reduced |
108400 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
109150 M-1 cm-1 |
|
|
| Codon Bias |
0.095 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.475 |
| Hydropathicity of Protein |
-0.403 |
| Aromaticity Score |
0.076 |
|
