Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
6.6% |
| Arg[R] |
23 |
4.3% |
| Asn[N] |
46 |
8.6% |
| Asp[D] |
48 |
9.0% |
| Cys[C] |
1 |
0.2% |
| Gln[Q] |
24 |
4.5% |
| Glu[E] |
68 |
12.7% |
| Gly[G] |
17 |
3.2% |
| His[H] |
7 |
1.3% |
| Ile[I] |
32 |
6.0% |
| Leu[L] |
47 |
8.8% |
| Lys[K] |
52 |
9.7% |
| Met[M] |
4 |
0.7% |
| Phe[F] |
16 |
3.0% |
| Pro[P] |
12 |
2.2% |
| Ser[S] |
43 |
8.1% |
| Thr[T] |
22 |
4.1% |
| Trp[W] |
3 |
0.6% |
| Tyr[Y] |
14 |
2.6% |
| Val[V] |
20 |
3.7% |
| Total Length |
534 |
|
| Instability index (II) |
52.96 |
| This classifies the protein as unstable |
|
| Carbon |
2644 |
| Hydrogen |
4204 |
| Nitrogen |
742 |
| Oxygen |
916 |
| Sulfur |
5 |
| Total number of atoms |
8511 |
| Formula = C2644H4204N742O916S5 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
37360 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
37360 M-1 cm-1 |
| assuming all Cys residues are reduced |
37360 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
37360 M-1 cm-1 |
|
|
| Codon Bias |
0.147 |
| Codon Adaptation Index |
0.206 |
| Frequency of Optimal Codons |
0.507 |
| Hydropathicity of Protein |
-1.034 |
| Aromaticity Score |
0.062 |
|
