Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
4.7% |
| Arg[R] |
27 |
4.0% |
| Asn[N] |
55 |
8.2% |
| Asp[D] |
39 |
5.8% |
| Cys[C] |
6 |
0.9% |
| Gln[Q] |
33 |
4.9% |
| Glu[E] |
47 |
7.0% |
| Gly[G] |
19 |
2.8% |
| His[H] |
16 |
2.4% |
| Ile[I] |
40 |
5.9% |
| Leu[L] |
83 |
12.3% |
| Lys[K] |
68 |
10.1% |
| Met[M] |
9 |
1.3% |
| Phe[F] |
18 |
2.7% |
| Pro[P] |
27 |
4.0% |
| Ser[S] |
66 |
9.8% |
| Thr[T] |
37 |
5.5% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
25 |
3.7% |
| Val[V] |
24 |
3.6% |
| Total Length |
674 |
|
| Instability index (II) |
46.66 |
| This classifies the protein as unstable |
|
| Carbon |
3398 |
| Hydrogen |
5464 |
| Nitrogen |
946 |
| Oxygen |
1063 |
| Sulfur |
15 |
| Total number of atoms |
10886 |
| Formula = C3398H5464N946O1063S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
54125 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
53750 M-1 cm-1 |
| assuming all Cys residues are reduced |
53750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
54125 M-1 cm-1 |
|
|
| Codon Bias |
0.001 |
| Codon Adaptation Index |
0.128 |
| Frequency of Optimal Codons |
0.409 |
| Hydropathicity of Protein |
-0.705 |
| Aromaticity Score |
0.068 |
|
